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Soluble Receptor in Mouse
Soluble receptors are a special class of proteins that are not anchored to cell membranes but exist freely in the extracellular space and are able to circulate in body fluids. These receptors play important roles in regulating a variety of biological functions, including immune responses, cell signaling, and tissue homeostasis. In mouse models, soluble receptors are particularly useful for studying human diseases and providing insight into complex signaling pathways.
Unlike membrane-bound receptors that interact directly with the cell surface, soluble receptors are usually released from cells into the blood or other body fluids by protein hydrolysis or secretion. These receptors can act as decoys or regulators that bind to their ligands but do not trigger the cellular responses normally associated with membrane-bound receptors. Their presence and concentration are influenced by a variety of factors, including inflammation, infection, and disease states.
In mouse models, soluble receptors are essential for studying a wide range of physiological and pathological processes. They can serve as biomarkers of disease progression, therapeutic response and immune system dysfunction. The spectrum of soluble receptors in mice tends to be similar to that of humans, making them ideal models for studying receptor-related diseases such as cancer, autoimmune diseases, cardiovascular diseases, and neurological disorders.
Mouse Soluble Receptor Panel at Creative Proteomics
Creative Proteomics offers a comprehensive and reliable Mouse Soluble Receptor Panel using Luminex xMAP Technology. This platform allows the simultaneous detection and quantification of multiple soluble receptors with high sensitivity and specificity, tailored to the needs of researchers investigating receptor-mediated signaling pathways. By providing precise and efficient analysis, we support the study of soluble receptors in mouse models, facilitating research into disease mechanisms, therapeutic development, and biomarker discovery.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Mouse
Analytes Detected
| Species | Specification | Protein Targets | Application | Price |
|---|---|---|---|---|
| Mouse | Mouse Soluble Receptor 10-plex Panel | EMMPRIN/CD147, Endoglin/CD105, ICAM-1/CD54, Osteopontin/OPN, RAGE/AGER, P-Selectin/CD62P, TIMP-1, TNF RI/TNFRSF1B, uPAR, MMP-2 | Suitable for studying biomarkers related to inflammation, immune response, tissue remodeling, and cancer. | +Inquiry |
Sensitivity (Detection Limits)
- EMMPRIN (CD147): 10–15 pg/mL
- Endoglin (CD105): 5–10 pg/mL
- ICAM-1 (CD54): 8–12 pg/mL
- Osteopontin (OPN): 10–15 pg/mL
- RAGE (AGER): 15–20 pg/mL
- P-Selectin (CD62P): 10–15 pg/mL
- TIMP-1: 12–18 pg/mL
- TNF RI (TNFRSF1B): 10–15 pg/mL
- uPAR: 5–10 pg/mL
- MMP-2: 10–15 pg/mL
Advantages of Mouse Soluble Receptor Luminex Assay
- Multiplexing Capability: The Luminex platform allows for the simultaneous detection of multiple soluble receptors in a single run, significantly improving throughput. This means you can analyze multiple biomarkers at once, reducing the number of experiments and sample requirements.
- High Sensitivity: The assay utilizes Luminex xMAP Technology, which is based on the detection of color-coded beads coated with specific antibodies. This high-sensitivity technology allows detection of soluble receptors at picogram or even femtogram levels, ensuring that even the most subtle changes in receptor levels can be detected.
- Low Sample Volume Requirement: The assay requires a relatively small volume of sample, making it ideal for precious or limited samples, such as those from animal models or clinical trials.
- Wide Dynamic Range: The Luminex Assay has an extensive dynamic range, allowing it to detect both low and high-abundance receptors in the same assay. This broad sensitivity ensures that the assay can be used for a variety of research applications, from basic biology to therapeutic development.
- Quick Turnaround Time: The Luminex platform enables high-throughput screening of soluble receptors, ensuring that you can receive your results in a timely manner to support rapid research and decision-making.
- Scalable for Different Applications: Whether you are conducting a large-scale study or a smaller, focused research project, the Mouse Soluble Receptor Panel can be scaled to meet the demands of your research protocol.
Sample Requirements for Mouse Soluble Receptor Luminex Panel
| Sample Type | Volume Required | Notes |
|---|---|---|
| Serum | 50 µL | Centrifuge to remove debris and keep on ice until analysis. |
| Plasma | 50 µL | Centrifuge at 3000 x g for 10 minutes, collect plasma fraction. |
| Cerebrospinal Fluid (CSF) | 30 µL | Use sterile containers and immediately store at -80°C if not analyzed immediately. |
| Tissue Lysates | 50-100 µL | Homogenize tissue in lysis buffer, centrifuge, and collect supernatant. |
| Cell Culture Supernatant | 50 µL | Filter culture supernatants to remove cells and debris. |
Application of Mouse Soluble Receptor Panel
- Immunology: Study immune response regulation and immune checkpoint signaling by quantifying soluble immune receptors.
- Inflammation: Monitor soluble receptors involved in inflammatory pathways and assess disease progression in inflammatory conditions.
- Cancer Research: Investigate biomarkers related to tumor progression, metastasis, and therapeutic responses.
- Tissue Remodeling: Analyze receptors involved in extracellular matrix remodeling, fibrosis, and wound healing.
- Neurology: Explore soluble receptors related to neuroinflammation and neurodegenerative diseases.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
| Protein Target | Description |
|---|---|
| EMMPRIN/CD147 | A receptor involved in extracellular matrix remodeling, immune response, and cancer progression. |
| Endoglin/CD105 | A co-receptor for TGF-β, involved in angiogenesis and vascular remodeling. |
| ICAM-1/CD54 | An adhesion molecule that plays a critical role in immune cell recruitment and inflammation. |
| Osteopontin/OPN | A glycoprotein involved in cell adhesion, inflammation, and tissue remodeling, commonly associated with cancer. |
| RAGE/AGER | A receptor that binds advanced glycation end-products (AGEs) and plays a role in inflammatory processes and neurodegenerative diseases. |
| P-Selectin/CD62P | An adhesion molecule important for leukocyte recruitment during inflammation and injury responses. |
| TIMP-1 | A tissue inhibitor of metalloproteinases, regulating extracellular matrix turnover and involved in tissue remodeling and cancer progression. |
| TNF RI/TNFRSF1B | A receptor for tumor necrosis factor (TNF), involved in mediating inflammation and apoptosis in various diseases. |
| uPAR | The urokinase-type plasminogen activator receptor, involved in cell migration, tissue remodeling, and cancer metastasis. |
| MMP-2 | Matrix metalloproteinase-2, an enzyme that degrades extracellular matrix components and plays a role in tissue remodeling, wound healing, and cancer metastasis. |
What are the key benefits of using the mouse soluble receptor panel over traditional single-plex assays?
- High-throughput multiplexing: The ability to analyze multiple receptors simultaneously in a single assay minimizes time, effort, and sample consumption. This is especially beneficial when working with limited or precious sample volumes.
- Increased efficiency: Performing multiple assays at once enhances experimental throughput and ensures consistency across different tests.
- Data integration: Multiplex assays provide a more comprehensive understanding of receptor dynamics by allowing the detection of various biomarkers in a single run, reducing potential variability and enhancing the depth of the data.
- Cost-effective: By consolidating multiple tests into one, the panel reduces the overall cost of experiments, especially in large-scale studies or when working with expensive reagents and limited sample material.
How do you ensure the quality and reproducibility of results in the mouse soluble receptor panel?
Creative Proteomics uses strict quality control protocols to ensure that the mouse soluble receptor panel delivers consistent and reliable results. These protocols include:
- Standardized Procedures: Each step of the assay, from sample preparation to data analysis, follows well-established, validated protocols to minimize experimental variability.
- Internal Controls: The panel incorporates positive and negative controls for each receptor, ensuring assay performance is consistently within expected parameters.
- Multiple Replicates: Data are collected from multiple replicates to assess reproducibility and accuracy, helping to confirm the reliability of the results.
- Expert Data Analysis: Our team of experts carefully reviews all results and provides insightful analysis to help interpret the data effectively.
Can I use the mouse soluble receptor panel for clinical or human samples?
The mouse soluble receptor panel is specifically optimized for use with mouse samples. If you are working with human or other species' samples, Creative Proteomics can provide similar panels designed for different species, including human, rat, or non-human primates. If you need a customized solution for clinical or human research, please reach out to our team to discuss your specific requirements.
