Human Non-Hematopoietic Soluble Receptor Panel Service

What is Human Non-Hematopoietic Soluble Receptor?

Human non-hematopoietic soluble receptors are a diverse group of proteins that play pivotal roles in cellular communication and regulation. Unlike hematopoietic receptors, which are primarily involved in blood cell function and immune responses, non-hematopoietic soluble receptors are distributed throughout various tissues and fluids, performing functions essential for maintaining homeostasis and facilitating intercellular signaling.

Human non-hematopoietic soluble receptors are characterized by their ability to exist in soluble forms within body fluids, such as blood plasma and cerebrospinal fluid. These receptors can bind to specific ligands, thereby modulating signaling pathways and influencing cellular responses. Key examples include receptors involved in metabolic regulation, growth factors, and neurotrophic factors.

The primary functions of these receptors include:

• Signal Transduction: They participate in transmitting signals from extracellular ligands to intracellular targets, affecting cell behavior and tissue function.
• Homeostasis Regulation: They help maintain internal balance by regulating physiological processes such as metabolism, growth, and immune responses.
• Disease Indicators: Elevated or decreased levels of these receptors can indicate pathological conditions, making them valuable biomarkers for various diseases.

Human Non-Hematopoietic Soluble Receptor Panel at Creative Proteomics

Creative Proteomics offers Human Non-Hematopoietic Soluble Receptor 15-plex Panel utilizing a magnetic bead-based Luminex multiplex assay. Luminex xMAP technology uses magnetic beads coated with specific antibodies to capture target receptors from biological samples. This multiplex assay approach allows for the concurrent measurement of multiple analytes, enhancing the efficiency and depth of analysis. The use of magnetic beads ensures high sensitivity and specificity, enabling the precise quantification of soluble receptors even at low concentrations.

Detection Method

Magnetic bead-based Luminex multiplex assay

Species

Human

Analytes Detected

Advantages of the Human Non-Hematopoietic Soluble Receptor Luminex Assay

• Multiplexing Capability: The Luminex assay enables the simultaneous analysis of up to 15 soluble receptors in a single sample, significantly enhancing throughput and efficiency. This multiplexing allows for comprehensive profiling of receptor interactions and signaling pathways without the need for multiple separate assays.
• High Sensitivity and Specificity: Utilizing color-coded magnetic beads and specific antibodies, the assay achieves high sensitivity and specificity, capable of detecting receptor levels as low as picograms per milliliter (pg/mL). This precision is crucial for detecting subtle changes in receptor levels associated with various diseases.
• Comprehensive Data with Broad Coverage: Covering a diverse range of 15 key receptors, including growth factors, adhesion molecules, and signaling proteins, the panel provides a holistic view of receptor profiles, essential for understanding complex biological processes like cancer progression, inflammation, and vascular biology.
• Efficiency and Cost-Effectiveness: By combining multiple receptor assays into one test, the Luminex approach reduces sample usage, testing time, and costs. Compared to conducting 15 separate ELISA tests, the multiplex assay offers significant savings in time and resources, making it ideal for large-scale studies and clinical diagnostics.
• Customizable Panels for Targeted Analysis: Creative Proteomics offers customizable panels, allowing researchers to select specific receptors or modify the panel according to their research needs. This flexibility ensures the assay can be tailored to specific hypotheses or study objectives.
• Reliable and Reproducible Results: With intra-assay precision typically ranging from 5% to 10% and inter-assay CVs generally below 15%, the assay delivers reliable and reproducible results, ensuring consistent performance across experiments and time points.
• Enhanced Sensitivity for Low-Abundance Targets: The assay's advanced detection system allows for the measurement of receptors present at very low concentrations, crucial for detecting early biomarkers or subtle changes in receptor expression, with detection limits as low as 1 pg/mL.

Sample Requirements for Human Non-Hematopoietic Soluble Receptor Luminex Panel

Why Analyze Human Non-Hematopoietic Soluble Receptors?

Cancer Biology

Tumor Progression and Metastasis: This panel is essential for studying the roles of specific receptors, such as ErbB2/Her2 and EPCAM/TROP1, in tumor development and the mechanisms underlying metastasis. It helps researchers explore how these receptors influence cancer cell behavior, including proliferation, invasion, and migration.

Angiogenesis Research: Receptors like VEGFR1/FIt-1 and VEGFR2/KDR are critical to studying the formation of new blood vessels, a process essential for tumor growth and survival. This panel enables researchers to quantify and understand the signaling pathways involved in angiogenesis.

Inflammation and Immune Response

Immune Signaling Pathways: The panel detects key receptors such as CD40/TNFRSF5 and E-Selectin/CD62E, which are involved in immune cell activation and migration. This allows researchers to dissect the signaling pathways that mediate inflammatory responses and their regulation in various contexts.

Chronic Inflammation Studies: By monitoring soluble receptor levels, the panel aids in understanding the molecular mechanisms that sustain chronic inflammation, contributing to research on diseases like arthritis and chronic inflammatory conditions.

Vascular Biology

Endothelial Cell Function: The panel includes receptors like ESAM and MCAM/CD146, which are important for studying endothelial cell interactions and their role in maintaining vascular integrity. This is crucial for understanding the dynamics of blood vessel formation, maintenance, and the effects of vascular dysfunction.

Vascular Remodeling: Research on vascular remodeling—how blood vessels adapt in response to physiological and pathological stimuli—benefits from the panel's ability to measure receptors that regulate these processes, aiding in the study of cardiovascular diseases and developmental biology.

Neuroscience

Neurodevelopmental Studies: The panel is instrumental in investigating how soluble receptors such as Syndecan-1/CD138 influence neural development, axon guidance, and synapse formation. These insights are vital for understanding brain development and neuroplasticity.

Neuroinflammation: The panel can be used to study the molecular underpinnings of neuroinflammation, which is implicated in various neurodegenerative disorders. By analyzing receptors involved in immune responses within the nervous system, researchers can explore potential targets for neuroprotective strategies.

Matrix Biology and Tissue Engineering

Extracellular Matrix (ECM) Interactions: The panel helps researchers study the interactions between cells and the ECM, focusing on receptors like Syndecan-4 and Thrombospondin-2, which play roles in cell adhesion, migration, and tissue organization.

Tissue Regeneration: In tissue engineering and regenerative medicine, understanding how receptors like Periostin/OSF-2 regulate tissue repair and remodeling is crucial. The panel provides data on the signaling pathways that control cell differentiation and tissue regeneration, supporting the development of new therapeutic strategies.

Developmental Biology

Receptor Signaling in Development: The panel facilitates research into the roles of soluble receptors during various stages of development. By measuring these receptors, researchers can gain insights into how signaling pathways govern processes like cell differentiation, organogenesis, and morphogenesis.

Gene Regulation and Expression: Understanding how receptor-mediated signaling affects gene expression during development is a key area of research. The panel allows for the analysis of these signaling cascades, contributing to the study of developmental disorders and congenital anomalies.

Signal Transduction Research

Pathway Analysis: The panel is highly valuable for dissecting complex signal transduction pathways. By quantifying multiple receptors simultaneously, researchers can map out the intricate networks that govern cell behavior and response to environmental stimuli.

Cross-Talk Between Pathways: The ability to analyze multiple receptors involved in different signaling pathways enables the study of cross-talk and integration between these pathways, which is essential for understanding how cells process multiple signals simultaneously.

In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!