Human MMP Panel Service

Description

Matrix metalloproteinases (MMP) are a group of metal (calcium and zinc)-dependent proteolytic enzymes that degrade the extracellular matrix. MMPs are involved in the release of cell surface active molecules (e.g., apoptotic ligands, growth factors), cleavage of cell surface receptors, modification of chemokines/cytokines, etc. MMPs are secreted intracellularly as the zymogen pro-MMP and are activated by a series of protease cascades to become active MMPs with hydrolytic capacity. Different MMPs are substrate specific and can be subdivided into gelatinases, collagenases, stromelysins, mesolysins, etc.

MMP-3 is capable of degrading or shearing a variety of extracellular matrix components, precursor proteins or precursor enzymes, including Collagen α-chains, Aggrecan, Laminin, Fibronectin, Elastin, Casein, α-1 Antitrypsin, Myelin Basic Protein, IL-1 β, IGFBP-3, pro-MMP-1, pro-MMP-7, pro-MMP-8, pro-MMP-9, pro-MMP-13, etc. MMP plays a key role in tumor development and multiple diseases, and its ability to disrupt histological barriers to tumor cell invasion, release E-calciferol (E-cadherin), promote tumor invasion and metastasis, and promote inflammatory responses has gained increasing attention in the study of tumors and other diseases. In addition, MMP-3 is also involved in a series of physiological and pathological processes such as tissue morphogenesis, damage repair, and inflammatory response, and plays an important role in the development of rheumatoid arthritis, atherosclerosis, and other diseases.

Creative Proteomics offers the Luminex Human MMP 3-plex/5-plex/9-plex Panel for quantifying MMP in serum, plasma, tissue/cell lysates, and culture supernatants. We base on Luminex xMAP technology to achieve ideal speed and sensitivity for simultaneous quantification of multiple analytes, significantly increasing productivity and saving valuable sample volumes.

Detection Method

Magnetic bead-based Luminex multiplex assay

Species

Human

Analytes Detected

Service Features

• 96-well plate design for batch detection of multiplexed factors up to 80 samples
• High sensitivity, as low as pg/mL
• Multi-factor assays performed with 50 μL liquid sample and 200 μg total protein
• Suitable for serum/plasma, culture supernatants, cells, tissue lysates
• Maximized information in a single sample and cross-analysis allowed between analytes
• Run your assay in just 3-5 hours
• Mass-calibrated standards for consistent results with every new lot of material

Application of MMP Panel

• Multiplexed protein assays
• Relative protein expression assays
• Detection of cytokine expression patterns
• Biomarker screening
• Identification of key factors
• Confirmation of biological processes