Description
Overweight and obesity are defined as abnormal or excessive accumulation of fat that may impair health. Obesity is a systemic disease that brings with it not only changes in body shape, but also a variety of diseases and complications that affect an individual's overall health. Common complications, such as hyperglycemia, hyperlipidemia and hypertension, are collectively referred to as "metabolic syndrome".
Common multifactorial obesity is likely the result of a combination of genetic, epigenetic and environmental factors. Obesity is now considered to be a chronic state of low-grade inflammation. Inflammation is one of the key processes in the development of insulin resistance, diabetes mellitus and related diseases. The development of metabolic syndrome and vascular disease is driven by humoral factors secreted by adipose tissue. These substances include leptin, adiponectin, resistin, TNF-α, IL-6, PAI-1, angiotensinogen, CRP (C-reactive protein), etc. Other cytokines such as adhesion molecules ICAM-1, VCAM-1 and E-selectin are also associated with endothelial cell activation and atherosclerosis in obese patients.
Creative Proteomics offers several optional Luminex Human Obesity-Related Cytokines 5-plex/9-plex/11-plex Panels to study cytokines and other marker proteins in serum, plasma and cell supernatants. Based on the Luminex xMAP technology, higher sensitivity and a wider range of assays are achieved, allowing simultaneous quantification of multiple analytes.
The following table lists the obesity-related factors that can be detected in human samples; Creative Proteomics also offers assays for other species. Inquiries are welcome.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Human
Analytes Detected
| Species | Specification | Protein Targets | Price |
|---|---|---|---|
| Human | Human Obesity-Related Cytokines 5-plex Panel | Adiponectin, Adipsin, NGAL/Lipocalin-2, PAI-1 (total), Resistin | +Inquiry |
| Human | Human Obesity-Related Cytokines 9-plex Panel | HGF, IL-1β, IL-6, IL-8/CXCL8, Insulin, Leptin, MCP-1/CCL2, NGF, TNFα | +Inquiry |
| Human | Human Obesity-Related Cytokines 11-plex Panel | Adiponectin, HGF, IL-1β, IL-6, IL-8/CXCL8, Leptin, MCP-1/CCL2, NGF, PAI-1(total), Resistin, TNFα | +Inquiry |
Service Features
- 96-well plate design for batch detection of multiplexed factors up to 80 samples
- High sensitivity, as low as pg/mL
- Multi-factor assays performed with 50 μL liquid sample and 200 μg total protein
- Suitable for serum/plasma, culture supernatants, cells, tissue lysates
- Maximized information in a single sample and cross-analysis allowed between analytes
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
Application of Obesity-Related Cytokines Hormone Panel
- Multiplexed protein assays
- Relative protein expression assays
- Detection of cytokine expression patterns
- Biomarker screening
- Identification of key factors
- Confirmation of biological processes