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What is Human CD8+ T Cell?
Human CD8+ T cells, also known as cytotoxic T lymphocytes (CTLs), are a critical component of the adaptive immune system. These cells are characterized by the expression of the CD8 glycoprotein on their surface, which distinguishes them from other T lymphocytes. CD8+ T cells are primarily responsible for identifying and eliminating cells that are infected by viruses, as well as cancerous cells.
The CD8 molecule acts as a co-receptor that enhances the interaction between T cells and antigen-presenting cells. This interaction is crucial for the activation and function of CD8+ T cells. Once activated, CD8+ T cells can directly induce apoptosis in target cells through the release of cytotoxic granules and the engagement of death receptors.
The quantitative and qualitative assessment of CD8+ T cells can provide insights into immune competence, disease progression, and therapeutic efficacy. For example, in cancer immunotherapy, the frequency and functional status of CD8+ T cells in tumors can predict treatment outcomes. Similarly, in chronic viral infections such as HIV, the exhaustion of CD8+ T cells is a hallmark of disease progression. Therefore, analyzing CD8+ T cells helps researchers and clinicians to monitor immune responses, develop targeted therapies, and assess the effectiveness of immunomodulatory treatments.
Human CD8+ T Cell Panel at Creative Proteomics
Creative Proteomics employs Luminex xMAP technology for conducting Human CD8+ T Cell 16 Plex Panel analyses. This cutting-edge technology allows for the multiplexed detection of numerous biomarkers within a single sample, providing high sensitivity and specificity. By using the Luminex xMAP platform, Creative Proteomics can deliver a detailed profile of CD8+ T cells, including markers related to activation, differentiation, and function. This capability facilitates a comprehensive understanding of CD8+ T cell dynamics, supporting advanced research and therapeutic strategies in immunology and disease treatment.
Detection Method
Magnetic bead-based Luminex multiplex assay
Species
Human
Analytes Detected
| Species | Specification | Protein Targets | Price |
|---|---|---|---|
| Human | Human CD8+ T Cell 16 Plex Panel | 4-1BB/TNFRSF9/CD137, CCL3/MIP-1 alpha, CCL4/MIP-1 beta, Fas/TNFRSF6/CD95, Fas Ligand/TNFSF6, Granzyme A, Granzyme B, IFN-gamma, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, TNF-alpha, GM-CSF | +Inquiry |
Advantages of the Human CD8+ T Cell Luminex Assay
- Multiplexing Capability: The Luminex assay allows for the simultaneous detection and quantification of multiple analytes (e.g., cytokines, chemokines, growth factors) in a single sample. This multiplexing capability saves time and conserves sample material, which is particularly valuable when working with limited sample volumes.
- High Sensitivity and Specificity: Luminex assays are known for their high sensitivity and specificity, allowing for the detection of low-abundance proteins that might not be easily detected using other methods like ELISA.
- Quantitative Analysis: The assay provides quantitative results, enabling the precise measurement of protein concentrations in samples. This is critical for comparing the levels of various cytokines or other markers in different conditions, such as healthy versus diseased states.
- High Throughput: The assay is well-suited for high-throughput applications, allowing researchers to analyze many samples in parallel. This is particularly advantageous in large-scale studies or clinical trials where a large number of samples need to be processed.
- Versatility: The Luminex platform can be adapted to measure a wide range of biomarkers, making it versatile for different research needs. It can be used to study immune responses, inflammation, cell signaling pathways, and more.
- Cost-Effective: By combining the analysis of multiple targets in one assay, Luminex reduces the need for multiple separate assays, which can be costly and time-consuming. This makes it a cost-effective solution for comprehensive protein analysis.
- Small Sample Volume Requirement: The assay requires only a small volume of sample, which is crucial when dealing with limited or precious biological materials, such as blood or tissue samples from patients.
- Data Reproducibility and Consistency: Luminex assays are known for producing highly reproducible and consistent data, which is essential for reliable experimental results and meaningful conclusions.
- Automation Potential: The Luminex system can be integrated with automated platforms, enhancing the efficiency and throughput of the assay. This is particularly useful in clinical laboratories or research settings with a high volume of samples.
- Broad Dynamic Range: The assay has a broad dynamic range, which allows for the detection of both high and low levels of proteins within the same sample, reducing the need for dilution and re-analysis.
Sample Requirements for Human CD8+ T Cell Luminex Panel
| Sample Type | Volume | Storage Conditions | Processing Timeframe |
|---|---|---|---|
| Peripheral Blood | 0.5 - 1 mL | 2-8°C, <24 hours | Process within 24 hours |
| Serum | 0.5 - 1 mL | -80°C for long-term | Freeze immediately after collection |
| Plasma | 0.5 - 1 mL | -80°C for long-term | Freeze immediately after collection |
| Cell Culture Supernatant | 0.5 - 1 mL | -80°C for long-term | Freeze immediately after collection |
| Isolated T Cells | 1x10^6 cells | 2-8°C, <24 hours | Process immediately after isolation |
Applications of Human CD8+ T Cell Panel Analysis
Cancer Immunotherapy: By analyzing CD8+ T cells, researchers can assess the effectiveness of immunotherapies, such as checkpoint inhibitors or CAR-T cell therapies. It helps identify specific T cell responses to tumors and monitor changes in T cell populations during treatment.
Vaccine Development: CD8+ T cell panels are crucial for evaluating vaccine-induced immune responses. They help determine how well a vaccine generates a cytotoxic T cell response against pathogens or cancer cells.
Autoimmune Disease Research: In autoimmune diseases, CD8+ T cells can contribute to tissue damage. Panel analysis helps in understanding their role in disease pathology and in developing targeted therapies.
Infection Monitoring: This analysis is used to study the immune response to viral infections, such as HIV or hepatitis. It helps track how CD8+ T cells respond to and control infections.
Transplantation: In organ transplantation, CD8+ T cell panels can be used to monitor graft rejection and tolerance, providing insights into the immune response against transplanted tissues.
Immunological Profiling: Detailed analysis of CD8+ T cell subsets and their functional states provides insights into overall immune health and helps in diagnosing and monitoring immune-related disorders.
Biomarker Discovery: Identifying specific CD8+ T cell markers can lead to new biomarkers for disease diagnosis, prognosis, and treatment response.
In addition to preconfigured panels, we also offer customized analysis services. You can customize your own panel through our customization tool, or directly email us the targets you are interested in. A professional will contact you to discuss the feasibility of customization. We look forward to working with you!
What is the turnaround time for receiving results from the Human CD8+ T Cell Panel analysis?
The turnaround time for receiving results typically ranges from 2 to 4 weeks, depending on the complexity of the analysis and the volume of samples. For expedited services, please contact us directly to discuss your requirements.
Can the Human CD8+ T Cell Panel be used for longitudinal studies or repeated measures?
Yes, the panel is well-suited for longitudinal studies. It allows for tracking changes in CD8+ T cell profiles over time, which is valuable for monitoring disease progression or treatment responses.
What measures are taken to ensure the accuracy and reliability of the assay results?
Several quality control measures are implemented to ensure accuracy and reliability:
- Calibration: Regular calibration of the Luminex equipment and reagents ensures accurate readings.
- Standard Curves: Each assay includes standard curves to quantify the concentration of biomarkers.
- Internal Controls: Positive and negative controls are run with each batch to validate the performance of the assay.
- Reproducibility Tests: Reproducibility is assessed through repeated testing of the same sample under identical conditions to ensure consistent results.
How do sample storage conditions affect the results of the Human CD8+ T Cell Panel?
Proper sample storage is crucial for maintaining the integrity of biomarkers:
- Peripheral Blood, Serum, Plasma, and Cell Culture Supernatant: Should be frozen at -80°C for long-term storage to prevent degradation. Improper handling, such as repeated freeze-thaw cycles, can affect the stability of biomarkers.
- Isolated T Cells: Should be processed immediately after isolation or stored at 2-8°C for up to 24 hours. Delayed processing can affect cell viability and functional status, impacting assay results.
How does the panel handle potential interferences or cross-reactivity between biomarkers?
The Luminex xMAP technology is designed to minimize cross-reactivity through the use of bead-based multiplexing. Each bead set is coated with distinct capture antibodies specific to different biomarkers. The spectral separation of these beads ensures that signals are detected individually, reducing the chance of cross-reactivity. Additionally, our assays include rigorous validation steps to detect and correct any potential interferences.